Increased immunofluorescence sensitivity using 532 nm laser excitation
نویسندگان
چکیده
منابع مشابه
Innovative Methodology Measurement of hemoglobin oxygen saturation using Raman microspectroscopy and 532-nm excitation
Torres Filho IP, Terner J, Pittman RN, Proffitt E, Ward KR. Measurement of hemoglobin oxygen saturation using Raman microspectroscopy and 532-nm excitation. J Appl Physiol 104: 1809–1817, 2008. First published March 27, 2008; doi:10.1152/japplphysiol.00025.2008.— The resonant Raman enhancement of hemoglobin (Hb) in the Q band region allows simultaneous identification of oxyand deoxy-Hb. The hem...
متن کاملMeasurement of hemoglobin oxygen saturation using Raman microspectroscopy and 532-nm excitation.
The resonant Raman enhancement of hemoglobin (Hb) in the Q band region allows simultaneous identification of oxy- and deoxy-Hb. The heme vibrational bands are well known at 532 nm, but the technique has never been used to determine microvascular Hb oxygen saturation (So(2)) in vivo. We implemented a system for in vivo noninvasive measurements of So(2). A laser light was focused onto areas of 15...
متن کاملComparison of laser iridotomy using short duration 532-nm Nd: YAG laser (PASCAL) vs conventional laser in dark irides.
AIM To evaluate the outcome of laser iridotomy using 532-nm Nd: YAG laser (PASCAL) with short pulse duration and Nd: YAG laser compared to conventional combined laser iridotomy. METHODS Retrospective, nonrandomized, comparative case series. Forty-five eyes of 34 patients underwent laser iridotomy. Twenty-two eyes underwent iridotomy using short duration PASCAL and Nd: YAG laser, and 23 eyes u...
متن کاملReverse intersystem crossing from a triplet state of rose bengal populated by sequential 532- q1064-nm laser excitation
A previously unstudied triplet state of rose bengal has been identified by near-infrared laser flash photolysis measureŽ . y16 2 ments. Its absorption peaks between 1050 and 1075 nm with a cross-section of 1.1"0.1 =10 cm in phosphate-buffered saline. This state, denoted as T , was further characterized through two-step laser-induced fluorescence measurements. The 2 photophysical parameters desc...
متن کاملEvaluating the effects of a 532-nm fiber-based KTP laser on transoral laser surgery supplies.
OBJECTIVE The KTP laser has become commonplace in transoral head and neck surgery. The interactions of this laser with commonly used supplies in transoral surgery have not been formally examined. This study evaluates the effects of the KTP laser on surgical supplies. STUDY DESIGN Experimental study. SETTING The study was conducted in an empty operating room at a university-affiliated medica...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2007
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.20358